| Congresso Brasileiro de Microbiologia 2023 | Resumo: 831-1 | ||||
Resumo:Salmonella enterica is a zoonotic pathogen of considerable concern to global human and animal health. Over 2500 serotypes have been described for Salmonella however, less than 100 serotypes account for most human infections. Non-typhoidal Salmonella infections are self-limiting diarrhoeal illness with low fatality cases, but in some cases can trigger an invasive disease with higher fatality cases when compared to non-invasive infection. A wide variety of domestic and wild animals, and a variety of food matrices can transmit Salmonella spp. to humans. The transfer often occurs when these microorganisms are introduced into food preparation areas, as well as through direct contact with infected animals or humans. Most of the data related to human salmonellosis in Brazil come from scientific research, since the Ministry of Health does not disclose annual reports as it occurs in North America and Europe. In Brazil, the National Reference Laboratory for Enteric Diseases, Oswaldo Cruz Institute – FIOCRUZ (NRL) receives Salmonella strains from Public Health Laboratories, Universities, Food Production Industries, and other Brazilian sectors for serotyping and genotyping. This allows tracing the profile of the serovars involved in human salmonellosis over time. Here, we report the top 10 Salmonella serovars isolated from human sources between 2011 and 2020. Isolates were received in NRL in Nutrient Agar and then inoculated in Costa and Vernin media, Lysine Iron Agar and sulphide-indole-motility and incubated at 37°C for 24 hours for confirmation as Salmonella enterica; serotyping was performed according to the Kauffman-White scheme. The prevalence and serovar diversity of Salmonella enterica from human sources were evaluated for 10 years. Until 2014 S. Enteritidis was the prevalent serovar in Brazil both in human and non-human sources. From 2015 onwards, S. Typhimurium which was the second increased its prevalence, occupying a prominent position and showing the occurrence of other serovars in human sourceS. However, it is necessary to evaluate the intraserovar diversity using subtyping methods such as PFGE and WGS. These will allow understanding the wide prevalence and diversity of Salmonella serovars, recognizing those prevalent in foodborne diseases. Palavras-chave: Salmonella, Foodborne disease, Serotyping Agência de fomento:Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, Oswaldo Cruz Foundation and Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro – FAPERJ | |||||